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| 产品名称： | pGAR1 CMCC 1775 |
|---|---|
| 商品货号： | TS163171 |
| Designations： | pGAR1 CMCC 1775 |
| Species： | Aspergillus awamori Nakazawa |
| Depositors： | Cetus Corp., A Belt, Cetus Corp. |
| Vector： | Construct size (kb): 7.300000190734863 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pACYC184 Intact vector size: 4.244 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: EcoRI HindIII BamHI SalI Polylinker sites: Construction: p15A, pSC101, Tn9 Host range: Escherichia coli Features (with orientation and position when available): marker(s): cmlR, tetR replicon: p15A Cross references: DNA Seq. Acc.: X06403 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Aspergillus awamori Gene name: glucoamylase Contains complete coding sequence?: U Strain: NRRL 15271 Type of DNA: genomic Insert end: EcoRI Insert end: EcoRI Insert size (kb): 3.4 Cross references: Insert lengths(kb): 3.400000095367432 Gene product: glucoamylase |
| Insert Size (kb)： | 3.400 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The insert contains the following restriction sites (approximate kb from the 5 end): SacI--0.4 (intron 1); BglII--1.0 (intron 3); PstI--1.1, 3.0 (exon 4, 3 flanking); BamHI--1.9 (exon 5); SalI--2.6 (3 flanking). Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 3.4; BamHI--4.1, 3.1; PstI--3.8, 1.8 (doublet). The insert detects a mRNA of 2.2 kb. |
| References： | Nunberg J, et al. Glucoamylase cDNA. US Patent 4,794,175 dated Dec 27 1988 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |