微信小陈
微信小章
产品简介
购买须知
| 产品名称： | KO#3 |
|---|---|
| 商品货号： | TS163206 |
| Designations： | KO#3 |
| GenBank Number： | M34353 |
| Species： | Homo sapiens, human |
| Depositors： | MH Wigler |
| Vector： | Construct size (kb): 4.800000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.78600001335144 Tissue: glioblastoma SW-1088 cell line Gene product: avian UR2 sarcoma virus oncogene (v-ros) homolog ROS Alleles: A1, A2, A1, A2 |
| Insert Size (kb)： | 1.786 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.8; BglII--4.8; HindIII--3.0, 1.6, 0.2; NotI--4.8; NotI/EcoRI--3.0, 1.8. The insert includes the following restriction sites (nt positions according to the published sequence): HindIII--9, 244; PstI--736; SacI--822; BamHI--851; BglII--1116. The insert extends from the 5 end of the published sequence to the EcoRI site at nt 1786, encoding the signal peptide and the amino terminal portion of the extracellular domain. The open reading frame extends from nt 207 to nt 7247. There is approximately 1 kb of 3 untranslated sequence which has not been fully characterized. Subcloned from lambdazN7. |
| References： | Birchmeier C, et al. Characterization of ROS1 cDNA from a human glioblastoma cell line. Proc. Natl. Acad. Sci. USA 87: 4799-4802, 1990. PubMed: 2352949 Michael H Wigler, personal communication |