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微信小章| 产品名称： | pBC-KC |
|---|---|
| 商品货号： | TS163248 |
| Designations： | pBC-KC |
| GenBank Number： | J04596 |
| Species： | Mus musculus, mouse |
| Depositors： | CD Stiles |
| Applications： | It is frequently used as a molecular marker for response to cellular growth factors. |
| Vector： | Construct size (kb): 3.799999952316284 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.800000011920929 Tissue: 3T3 (clone A31) cell line Gene product: GRO1 oncogene (melanoma growth stimulating activity, alpha)(growth regulated (alpha), growth regulated (group I)) Mgsa |
| Insert Size (kb)： | 0.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--2.8, 0.84; EcoRI--3.85; HindIII--3.85. KC is one of several competence genes (including JB, JE) that are induced with the addition of platelet-derived growth factor. It is frequently used as a molecular marker for response to cellular growth factors. Detects a mRNA of 1.2 kb after PDGF induction. The level of induction is increased by cycloheximide treatment. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Cochran BH, et al. Molecular cloning of gene sequences regulated by platelet-derived growth factor. Cell 33: 939-947, 1983. PubMed: 6872001 Sakaguchi AY, et al. Mouse melanoma growth stimulator activity gene (Mgsa) is polymorphic and syntenic with the W, patch, rumpwhite, and recessive spotting loci on chromosome 5. Genomics 5: 629-632, 1989. PubMed: 2575589 Oquendo P, et al. The platelet-derived growth factor-inducible KC gene encodes a secretory protein related to platelet alpha-granule proteins. J. Biol. Chem. 264: 4133-4137, 1989. PubMed: 2917992 |