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| 产品名称： | pKBY2 |
|---|---|
| 商品货号： | TS163265 |
| Designations： | pKBY2 |
| Depositors： | Regents of the University of California, WE Timberlake, Regents of the University of California |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 |
| Vector Information： | Size (kb): 9.8999996185302730 Vector: pKBY2 (cosmid) Construction: pBR329, Aspergillus nidulans trpC, pJB8 cos Marker(s):ampR,cmlR,trpC+ Construct size (kb): 9.899999618530273 Features: marker(s): ampR, cmlR, trpC+ replicon: pMB1 enhancer: none |
| Applications： | shuttle vector vector for constructing genomic libraries |
| Comments： | IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. This is a cosmid vector for isolating genes by complementation of mutations in Aspergillus nidulans. It can also be used for library construction. A 2.1 kb HindIII/SalI fragment (pJB8) containing lambda cos was ligated to HindIII/SalI-digested pBR329. A 4.1 kb XhoI fragment with wild-type Aspergillus nidulans trpC was ligated into the SalI, and a BamHI linker into the NruI site of pBR329. The BamHI site was removed from trpC by sodium bisulfite mutagenesis. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Yelton MM, et al. A cosmid for selecting genes by complementation in Aspergillus nidulans: selection of the developmentally regulated yA locus. Proc. Natl. Acad. Sci. USA 82: 834-838, 1985. |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |