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| 产品名称： | pE4 |
|---|---|
| 商品货号： | TS163305 |
| Designations： | pE4 |
| GenBank Number： | M10988 X51416 |
| Species： | Homo sapiens, human |
| Depositors： | Cetus Corp., A Belt, Cetus Corp. |
| Vector： | Construct size (kb): 4.80
Name of vector: pcDV1 Intact vector size: 3.000 Type of vector: plasmid Cloning sites: SalI ClaI HindIII EcoRI KpnI Polylinker sites: Construction: pBR322, SV40 Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): enhancer: none marker(s): ampR promoter: none replicon: pMB1 terminator: SV40 late Cross references: |
| Insert： | DNA: cDNA
Genome: human Gene symbol: TNFA Genomic copy number: unique Gene name: tumor necrosis factor, alpha (cachectin) Contains complete coding sequence?: U Chromosome: 6; Localization: 6 p21.3 Tissue: HL-60 cell line (promyelocytic leukemia) Type of DNA: cDNA Insert 5 end: Modification: GC Insert 3 end: Modification: AT Insert size (kb): 1.585 Cross references: DNA Seq. Acc.: M10988 Insert lengths(kb): 1.59
Tissue: HL-60 cell line (promyelocytic leukemia)
Gene product: tumor necrosis factor, alpha (cachectin) TNFA |
| Insert Size (kb)： | 1.585 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert contains recognition sites for the following enzymes: HgiA1 (2), BglI (2), NarI, AvaI, PvuII (2), HindIII, EcoRI, PstI, StuI (2). A PstI digest will release a 1.1 kb fragment which contains the TNF coding sequence plus some vector sequence at the 5 end and some 3 untranslated sequence. Restriction digests of the clone give the following sizes (kb): PstI--2.4, 1.4, 1.1; HindIII--3.4, 1.5; BamHI--3.1, 1.8; EcoRI--4.6; EcoRI/PstI--2.4, 1.5, 1.0; EcoRI/BamHI--3.1, 1.1, 0.7; HindIII/PstI--2.4, 0.9, 0.8, 0.5; BamHI/HindIII--2.7, 1.0, 0.74, 0.47; StuI--3.5, 2.2, 0.25. |
| References： | Mark DF, et al. Human tumor necrosis factor. US Patent 4,677,063 dated Jun 30 1987 Mark DF, et al. Human tumor necrosis factor. US Patent 4,677,064 dated Jun 30 1987 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |