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| 产品名称： | pNF2 |
|---|---|
| 商品货号： | TS163327 |
| Designations： | pNF2 |
| Depositors： | PA Scolnik |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.8000001907348630 Vector: pNF2 (plasmid) Promoters: Promoter nifH Construction: pBR322, nifH, str/spcR Marker(s):ampR,spcR,strR Construct size (kb): 7.800000190734863 Features: marker(s): ampR, spcR, strR promoter: nifH replicon: pMB1 terminator: none |
| Applications： | expression vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): SalI--8.2; BamHI--8.2; EcoRI/PstI--4.2, 3.6. The spcR marker is expressed in R. capsulatus. A wide range of transcription levels can be obtained by varying the nitrogen source in the medium. Transcription can be immediately stopped by the introduction of oxygen and ammonia. A Rhodobacter capsulatus expression vector with transcription of the insert under the control of the nifH promoter. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Pollock D, et al. Transcription of the rhodobacter capsulatus nifHDK operon is modulated by the nitrogen source. Construction of plasmid expression vectors based on the nifHDK promoter. Gene 65: 269-275, 1988. PubMed: 3410321 |
| Shipped： | freeze-dried |
| Shipping Information： | Shipped: xa0Freeze dried E. coli containing the plasmid |