| 产品名称： |
Fibroblast Growth Kit-Serum-free |
| 商品货号： |
TS163411 |
| Biosafety Level： |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： |
frozen 1 kit |
| Storage Conditions： |
-20° (or -70°C for long-term storage) |
| Comments： |
The kit contains components that when added to the fibroblst basal medium ATCC PCS-201-030 create a complete serum-free culture environment for fibroblasts. The final concentration for each component is as follows:
- HLL Supplement: HSA 500 µg/mL, linoleic acid 0.6 mM, lecithin 0.6 µg/mL
- L-Glutamine: 7.5 mM
- rh FGF basic: 5 ng/mL
- rh EGF/TGF-1 Supplements: 5 ng/mL and 30 pg/mL, respectively
- rh Insulin: 5 µg/mL
- Hydrocortisone: 1 µg/mL
- Ascorbic acid: 50 µg/mL
|
| Complete Growth Medium： |
-
Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
-
Thaw the components of the growth kit just prior to adding to the basal medium. It is necessary to warm the L-glutamine component in a 37°C water bath, and shake to dissolve any precipitates, prior to adding to the basal medium.
-
Obtain one bottle of Fibroblast Basal Medium (480 mL) from cold storage.
-
Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
-
Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the volume of each growth kit component, as indicated in Table 1 or 2, to the bottle of basal medium using a separate sterile pipette for each transfer.
Table 1. If using the Fibroblast Growth Kit–Serum-Free (ATCC® PCS-201-040), add the indicated volume for each component in the order shown.
|
Component |
| Volume： |
Volume |
| Sterility Tests： |
Final Concentration |
| Functional Tests： |
L-glutamine |
| C of A： |
18.75 mL |
|
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