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微信小章| 产品名称： | Adeno-associated virus 4 |
|---|---|
| 商品货号： | TS163480 |
| Classification： | Parvoviridae, Dependovirus, Adeno-associated virus-4 |
| Deposited As： | Adeno-associated virus type 4 |
| Agent： | Adeno-associated virus 4 |
| Strain： | none |
| Positive for Mycoplasma： | Yes |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Monkey cells infected with SV-15 virus |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | Depends upon adenovirus for replication. *Helper virus may be inactivated at 56C for 30 min. |
| Effect on Host： | Cytopathic effect: AAV produces no CPE. Helper adenovirus induces refractile rounding and cell sloughing. AAV produces no CPE.xa0 Helper adenovirus induces refractile rounding and cell sloughing.xa0 Non-cytopathic without helper virus |
| Recommended Host： | monkey kidney cells; suckling mouse Recommended Host:xa0 LLC-MK2 (ATCC® CCL-7.1TM) |
| Growth Conditions： | Temperature: 37.0°C Duration: Evidence of virus growth can be expected within 2-3 days. For best results cells should be 24-48 hours old and 80%-90% confluent (not 100%) at the time of infection. |
| Name of Depositor： | WP Parks |
| Source： | Monkey cells infected with SV-15 virus |
| References： | Chiorini JA, et al. Cloning of adeno-associated virus type 4 (AAV4) and generation of recombinant AAV4 particles. J. Virol. 71: 6823-6833, 1997. PubMed: 9261407 Parks WP, et al. Physical assay and growth cycle studies of a defective adeno-satellite. J. Virol. 1: 171-180, 1967. PubMed: 4990036 |
| Cross References： | Nucleotide (GenBank) : U89790 Adeno-associated virus 4, complete genome. Nucleotide (GenBank) : NC_001829 Adeno-associated virus 4, complete genome. |