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| 产品名称： | uPAR/Bluescript |
|---|---|
| 商品货号： | TS163535 |
| Designations： | uPAR/Bluescript |
| Species： | Homo sapiens, human |
| Depositors： | E Kruithof |
| Vector： | Construct size (kb): 4.099999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.11300003528595 Tissue: fibroblast GM637 cell line, SV40-transformed Gene product: plasminogen activator, urokinase, receptor(activation antigen Mo3, urokinase plasminogen activator surface receptor) Mo3 |
| Insert Size (kb)： | 1.113 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/XbaI--3.0, 1.l; PstI--3.8, 0.3; HindIII--4.1; BamHI--3.5, 0.6; SmaI--4.1. The insert contains the following restriction sites (nt position according to the published sequence): PstI--181, 449; BamHI--498, 1083; SmaI--576. Corresponds to nt 31 - 1144 of the published sequence, and contains the complete coding region (nt 47 - 1054). Transcription from the T7 promoter gives the sense strand. |
| References： | Roldan AL, et al. Cloning and expression of the receptor for human urokinase plasminogen activator, a central molecule in cell surface, plasmin dependent proteolysis. EMBO J. 9: 467-474, 1990. PubMed: 1689240 Egbert Kruithof, personal communication |