微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pRR2119 |
|---|---|
| 商品货号： | TS163553 |
| Designations： | pRR2119 |
| GenBank Number： | K01999 |
| Species： | Rhodospirillum rubrum (Esmarch) Molisch |
| Depositors： | CR Somerville |
| Applications： | produces protein ribulose-1,5-bisphosphate carboxylase oxygenase |
| Vector： | Construct size (kb): 6.0 |
| Insert： | DNA: genomic Insert lengths(kb): 2.40 Gene product: ribulose-1,5-bisphosphate carboxylase oxygenase RuBP Target Gene: ribulose-1,5-bisphosphate carboxylase oxygenase |
| Insert Size (kb)： | 2.400 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Shipped: xa0Freeze dried E. coli containing the plasmid |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 2.5, 0.6; PstI--3.1, 2.0, 0.8; SalI--5.6, 0.3. The insert contains the following restriction sites (kb from the EcoRI site 10 bp 5 to the BamHI/Sau3AI junction): SalI--0.26, 0.52, 0.85; PstI--1.0, 1.8. Expression is regulated by the lac promoter, and is low in the absence of inducer. |
| References： | Somerville CR, Somerville SC. Cloning and expression of the Rhodospirillum rubrum ribulosebisphosphate carboxylase gene in E. coli. Mol. Gen. Genet. 193: 214-219, 1984. Nargang F, et al. Nucleotide sequence of the ribulosebisphosphate carboxylase gene from Rhodospirillum rubrum. Mol. Gen. Genet. 193: 220-224, 1984. |