微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pSELECT-1 pALTER-1 |
|---|---|
| 商品货号： | TS163612 |
| Designations： | pSELECT-1 pALTER-1 |
| Depositors： | Promega Corporation, JC Grosch, Promega Corporation |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.6799998283386230 Vector: pSELECT-1 (phagemid) Promoters: Promoter T7 Construction: pBR322, pGEM-3Zf(+) Marker(s):tetR Construct size (kb): 5.679999828338623 Features: insert detection: lacZ marker(s): tetR promoter: lac, SP6, T7 replicon: pMB1, f1 |
| Applications： | vector containing primer sites useful for sequencing vector permitting RNA synthesis in vitro vector permitting visual detection of recombinants vector useful for in-vitro mutagenesis |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.2; BamHI--6.2; HindIII--6.2. This vector contains the following unique restriction sites: ClaI, EcoRV, StyI, ScaI and AatII. Useful for in vitro mutagenesis experiments. Encodes the beta-galactosidase alpha peptide, permitting blue-white visual detection. Can be used to produce ssDNA. Contains promoters for in vitro RNA synthesis and priming sites useful for sequencing. Contains an inactive ampicillin resistance gene which can be reverted to functional expression by a repair oligonucleotide. |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Promega Corporation, personal communication |
| Shipped： | frozen |
| Shipping Information： | Shipped: xa0Freeze dried E. coli containing the plasmid |