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| 产品名称： | YEpGAL10-FLP YEp51-FLP |
|---|---|
| 商品货号： | TS163698 |
| Designations： | YEpGAL10-FLP YEp51-FLP |
| Species： | Saccharomyces cerevisiae, 2 micron circle |
| Depositors： | JR Broach |
| Applications： | in another host, produces protein recombinase, site-specific |
| Vector： | Construct size (kb): 7.699999809265137 DESCRIPTION OF VECTOR COMPONENT: Name of vector: YEp51 Intact vector size: 6.800 Type of vector: plasmid Vector end: SalI Vector end: HindIII Cloning sites: HindIII SalI BamHI Polylinker sites: Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli Features (with orientation and position when available): marker(s): ampR, LEU2 replicon: pMB1, 2 micron Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Saccharomyces cerevisiae, 2 micron circle Gene symbol: FLP Genomic copy number: unique Gene name: recombinase, site-specific Contains complete coding sequence?: U Type of DNA: genomic Insert end: HaeIII Insert end: HindIII Insert size (kb): 0.9 Cross references: Insert lengths(kb): 0.8999999761581421 Gene product: recombinase, site-specific FLP Target Gene: recombinase, site-specific |
| Insert Size (kb)： | 0.900 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.4, 2.2 (doublet); HindIII/SalI--7.0, 0.9; SalI--7.8; PstI--6.8, 1.05; XbaI--7.8. Plasmid to eliminate 2 micron circle plasmids and generate cir0 strains by overexpression of FLP. |
| References： | Rose AB, Broach JR. Propagation and expression of cloned genes in yeast: 2-micron circle-based vectors. Methods Enzymol. 185: 234-279, 1990. PubMed: 2199781 Volkert FC, Broach JR. Site-specific recombination promotes plasmid amplification in yeast. Cell 46: 541-550, 1986. PubMed: 3524855 |