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| 产品名称： | pDP1648 |
|---|---|
| 商品货号： | TS163750 |
| Designations： | pDP1648 |
| GenBank Number： | U65918 |
| Species： | Homo sapiens, human |
| Depositors： | DC Page, R Reijo, LG Brown, R Saxena |
| Vector： | Construct size (kb): 5.199999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.200000047683716 Tissue: testis Gene product: DAZ homologue DAZH |
| Insert Size (kb)： | 2.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/XbaI--3.2, 2.2; NdeI--5.3; SspI/XhoI--2.2, 1.0, 0.66 (doublet), 0.55, 0.28. Insert contains the following restriction sites (approximate kb from the 5 end): EcoRV--0.76; HindIII--0.39, 1.82; NdeI--1.68; NcoI--0.52; PvuII--0.28, 0.85; XhoI--0.18. Digestion with XhoI and SspI released a 0.95 kb fragment containing the entire open reading frame. |
| References： | Saxena R, et al. The DAZ gene cluster on the human Y chromosome arose from an autosomal gene that was transposed, repeatedly amplified and pruned. Nat. Genet. 14: 292-299, 1996. PubMed: 8896558 David C Page, personal communication |