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微信小章| 产品名称： | 42 |
|---|---|
| 商品货号： | TS163772 |
| Designations： | 42 |
| GenBank Number： | M87857 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 40.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pJB8 Intact vector size: 5.400 Type of vector: cosmid Vector end: BamHI Vector end: BamHI Cloning sites: BamHI EcoRI Polylinker sites: Construction: HomerI Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR replicon: pMB1 Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: LGALS2 Other gene symbols: GALBP Genomic copy number: unique Gene name: lectin, galactoside-binding, soluble, 2 (galectin 2) Contains complete coding sequence?: U Chromosome: 22; Localization: 22 q12-q13 Tissue: leukocyte Type of DNA: genomic Insert end: MboI Insert end: MboI Insert size (kb): 35.0 Cross references: DNA Seq. Acc.: M87857 DNA Seq. Acc.: M87858 DNA Seq. Acc.: M87859 DNA Seq. Acc.: M87860 Insert lengths(kb): 35.0 Tissue: leukocyte Gene product: lectin, galactoside-binding, soluble, 2 (galectin 2) GALBP |
| Insert Size (kb)： | 35.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--15.0, 8.5, 7.5, 2.7, 1.85, 1.8, 1.05, 1.0, 0.55; BamHI/EcoRI--9.5, 7.5, 5.8, 3.0, 2.5, 1.85, 1.8, 1.6, 1.5, 1.05, 1.0, 0.75, 0.55, 0.55; HindIII/EcoRI--12.5, 4.55, 3.6, 3.0, 2.9, 2.7, 2.5, 1.75, 1.7, 1.2, 1.08, 1.08, 1.0, 0.7, 0.55; KpnI/EcoRI--15.0, 8.5, 7.5, 2.7, 1.85, 1.8, 1.05, 1.0, 0.55; SalI/EcoRI--15.0, 7.5, 5.0, 2.7, 2.5, 2.5, 1.85, 1.8, 1.05, 1.0, 0.55. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Contains the complete gene (4 exons). |
| References： | Lau YF, Kan YW. Versatile cosmid vectors for the isolation, expression, and rescue of gene sequences: studies with the human alpha-globin cluster. Proc. Natl. Acad. Sci. USA 80: 5225-5229, 1983. PubMed: 6310566 Gitt MA, et al. Isolation and expression of a gene encoding L-14-II, a new human soluble lactose-binding lectin. J. Biol. Chem. 267: 10601-10606, 1992. PubMed: 1375225 Gitt MA, Barondes SH. Genomic sequence and organization of two members of a human lectin gene family. Biochemistry 30: 82-89, 1991. PubMed: 1988031 |