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| 产品名称： | pYAC3 |
|---|---|
| 商品货号： | TS163773 |
| Designations： | pYAC3 |
| Depositors： | DT Burke |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 11.6999998092651400 Vector: pYAC3 (YAC) Promoters: Promoter none Construction: pBR322, A240pI Marker(s):TRP1,URA3,ampR,HIS3,SUP4ochre Construct size (kb): 11.69999980926514 Features: marker(s): ampR, URA3, TRP1, SUP4ochre, HIS3 promoter: none replicon: pMB1, ARS1 centromere: CEN4 |
| Applications： | YL-type (YAC) shuttle vector shuttle vector vector for constructing genomic libraries |
| Comments： | The SnaBI site, in the intron of SUP4ochre, inactivates SUP4ochre. A yeast artificial chromosome vector. pYAC3 was constructed from pYAC2 by replacing the SmaI site in the SUP4 intron with the SnaBI site, and eliminating the SfiI and NotI sites flanking the SUP4 gene. The HIS3 sequence is removed when producing the telomers by BamHI digestion. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Burke DT, et al. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science 236: 806-812, 1987. PubMed: 3033825 |
| Shipped： | freeze-dried |