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| 产品名称： | CsCMV28 pCsCMV28 |
|---|---|
| 商品货号： | TS163839 |
| Designations： | CsCMV28 pCsCMV28 |
| GenBank Number： | U23414 |
| Species： | cassava common mosaic virus |
| Depositors： | LA Calvert |
| Vector： | Construct size (kb): 6.349999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 3.400000095367432 Gene product: genome, partial, 3 end |
| Insert Size (kb)： | 3.400 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.2, 1.4; PstI--6.0, 0.8; XbaI--3.6, 3.0. Insert contains the following restriction sites (approximate kb from the 5 end): BamHI--1.95, 3.26; BglII--2.19; EcoRI--1.17; EcoRV--0.69; HindIII--1.99, 2.48; KpnI--0.89, 1.18, 2.76; NcoI--0.03, 1.43; PstI--2.74; PvuII--2.30, 2.72; SacI--0.59; SmaI--0.45, 2.29; XbaI--0.05; XhoI--0.14. Insert contains the complete coding sequence for ORFs 2-5 and includes approximately 30 bp polyadenylation. Virus was propagated in Nicotiana benthamiana. |
| Classification： | Potexvirus |
| References： | Calvert LA, et al. Characterization of cassava common mosaic virus and a defective RNA species. J. Gen. Virol. 77: 525-530, 1996. PubMed: 8601791 |