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| 产品名称： | pCu424CTR1-CYC1 |
|---|---|
| 商品货号： | TS163859 |
| Designations： | pCu424CTR1-CYC1 |
| Depositors： | S Labbe, DJ Thiele |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.2280001640319820 Vector: pCu424CTR1-CYC1 (phagemid) Promoters: Promoter for expression CTR1-CYC1 Construction: pRS424, CTR1-CYC1 promoter Marker(s):TRP1,ampR Construct size (kb): 6.228000164031982 Features: marker(s): TRP1 marker(s): ampR promoter for expression: CTR1-CYC1 replicon: 2 micron replicon: f1 replicon: pMB1 MCS: XhoI...SpeI terminator: CYC1 |
| Applications： | cloning vector |
| Comments： | Restriction digests of the vector give the following sizes (kb): SacI--6.2; SacI/SpeI--5.9, 0.4. CTR3 and CTR1 are the copper transporter promoters and are repressed by the presence of copper and highly activated under copper-starvation conditions. The time course of copper-starvation activation and copper repression of a reporter gene is rapid. |
| References： | Labbe S, Thiele DJ. Copper ion inducible and repressible promoter systems in yeast. Methods Enzymol. 306: 145-153, 1999. PubMed: 10432452 |
| Related Products： | component of:ATCC 87778 |
| Shipped： | dried |