微信小陈
微信小章
产品简介
购买须知
| 产品名称： | YIp365 |
|---|---|
| 商品货号： | TS163864 |
| Designations： | YIp365 |
| Depositors： | CJ Lusty |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.0000000000000000 Vector: YIp365 (plasmid) Promoters: Promoter none Construction: YEp365 (ATCC 37730), YIp351 Marker(s):LEU2,ampR Construct size (kb): 7.0 Features: insert detection: lacZ marker(s): ampR, LEU2 promoter: none replicon: pMB1 |
| Applications： | YI-type (integrating) shuttle vector promoter-cloning vector shuttle vector |
| Comments： | The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): EcoRI-3; SmaI-1; BamHI-1; SalI-1; PstI-2; HindIII-3. The EcoRI site is not unique. Restriction digests of the clone give the following sizes (kb): EcoRI--4.1, 2.9; SalI--7.0; PstI--7.0; HindIII--7.0. One of 3 promoter-cloning, YI type shuttle vectors (ATCC 37746 - 37748) with LEU2 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame. The sequence and reading frame of the multiple cloning sequence is: 5 G AAT TCC CGG GGA TCC GTC GAC CTG CAG CCA AGC TTC GAT CCC 3. The sequence begins with the first nucleotide of the MCS and ends with the CCC for amino acid 8 of beta-galactosidase. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Myers AM, et al. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. Gene 45: 299-310, 1986. PubMed: 3026915 |
| Shipped： | freeze-dried |