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| 产品名称： | EBO-pCD-Leu2 |
|---|---|
| 商品货号： | TS163872 |
| Designations： | EBO-pCD-Leu2 |
| Species： | Homo sapiens, human |
| Depositors： | RF Margolskee |
| Applications： | DNA from secondary transfectants was used to isolate the clone. |
| Vector： | Construct size (kb): 12.89999961853027 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.299999952316284 Tissue: JM cell line DNA transferred into J107 cells Gene product: antigen CD8A (p32)( CD8 antigen, alpha-chain, T-cell antigen Leu2, T-cell surface glycoprotein CD8 alpha chain, T8 T-cell antigen) CD8 |
| Insert Size (kb)： | 2.300 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Digestion with BamHI yields 2 vector fragments (5.4, 5.2 kb) and the 2.3 kb insert (+ some pcD). Restriction digests of the clone give the following sizes (kb): NotI--uncut; BssHII--13.0; SfiI--7.6, 5.4; PstI--7.4, 3.9, 1.5; SacI--13.0; BamHI--5.2 doublet, 2.3; HindIII--13.0; BglII--13.0. Vector contains EBV oriP and EBNA-1 to ensure replication in absence of EBNA. Maintained as episomes as 2-10 copies per lymphoblastoid cell; stable as long as selection is applied. J107 cells were transformed with DNA from the T-lymphoma JM cell line. DNA from secondary transfectants was used to isolate the clone. |
| References： | Margolskee RF, et al. Epstein-Barr virus shuttle vector for stable episomal replication of cDNA expression libraries in human cells. Mol. Cell. Biol. 8: 2837-2847, 1988. PubMed: 2841588 |