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Neospongiococcum excentricum Deason and Bold

货号 TS163933
中文名称 null
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产品名称: Neospongiococcum excentricum Deason and Bold
商品货号: TS163933
Strain Designations: HZ-1236/437
Application:
produces zeaxanthin and other carotenoids
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation:
mutant derived from ATCC 40335
Product Format: frozen
Type Strain: no
Disclosure: This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Medium: ATCC® Medium 2085: YEP medium
Growth Conditions:
Temperature: 30.0°C
Duration: axenic
Protocol: This strain is routinely shipped as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Thaw the ampule and aseptically transfer the entire contents to 5 ml of ATCC medium 200 in a 16 x 125 mm screw-capped test tube. DO NOT distribute the thawed material to a larger volume of medium. It is essential to first establish the culture in a small volume. Screw the cap on tightly, loosen it by one half turn, and incubate the tube on a horizontal slant (5-15 degree slant) at 30C. The culture should be ready to subculture in 5-7 days. To subculture, crew the cap on tightly, agitate the tube vigorously, and aseptically transfer a 0.1 ml aliquot to 5 ml of ATCC medium 2085. Incubate as above.
Subcultivation:
Protocol: This strain is routinely shipped as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Thaw the ampule and aseptically transfer the entire contents to 5 ml of ATCC medium 200 in a 16 x 125 mm screw-capped test tube. DO NOT distribute the thawed material to a larger volume of medium. It is essential to first establish the culture in a small volume. Screw the cap on tightly, loosen it by one half turn, and incubate the tube on a horizontal slant (5-15 degree slant) at 30C. The culture should be ready to subculture in 5-7 days. To subculture, crew the cap on tightly, agitate the tube vigorously, and aseptically transfer a 0.1 ml aliquot to 5 ml of ATCC medium 2085. Incubate as above.
Name of Depositor: Coors BioTech, Inc., SA Orndorff, Coors BioTech, Inc.
U.S. Patent Number:
U.S. Patent:
References:

Orndorff SA, et al. Zeaxanthin producing strains of Neospongiococcum excentricum. US Patent 5,360,730 dated Nov 1 1994