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| 产品名称： | pBact-myc |
|---|---|
| 商品货号： | TS163941 |
| Designations： | pBact-myc |
| Depositors： | A Matus |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 5.6180000305175780 DESCRIPTION OF VECTOR: Intact vector size: 5.618 Type of vector: plasmid Cloning sites: EcoRI ClaI Polylinker sites: HindIII NcoI EcoRI ClaI HindIII Construction: pBact16, pUC18, c-myc Host range: Escherichia coli; mammalian cells Features (with orientation and position when available): promoter: beta-actin, -> restriction site: HindIII other: Kozak consensus sequence restriction site: NcoI other: c-myc epitope sequence, -> restriction site: EcoRI restriction site: ClaI restriction site: HindIII terminator: SV40 polyadenylation replicon: pMB1 marker(s): ampR, Vector: pBact-myc (plasmid) Promoters: Promoter beta-actin Construction: pBact16, pUC18, c-myc Marker(s):ampR Construct size (kb): 5.618000030517578 Features: marker(s): ampR other: Kozak consensus sequence other: c-myc epitope sequence promoter: beta-actin replicon: pMB1 restriction site: ClaI restriction site: EcoRI restriction site: HindIII restriction site: NcoI terminator: SV40 polyadenylation |
| Applications： | expression vector shuttle vector vector permitting construction of fusion proteins |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--5.6, PstI--4.8, 0.45, 0.35; EcoRI--5.6. Shuttle expression vector allowing production of a fusion protein containing an amino terminal c-myc epitope tag (FQKLISEEDLN), that can be used for immunological detection of the recombinant protein in transfected cells. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Cravchik A, Matus A. A novel strategy for the immunological tagging of cDNA constructs. Gene 137: 139-143, 1993. PubMed: 7506688 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |