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| 产品名称： | pK |
|---|---|
| 商品货号： | TS164020 |
| Designations： | pK |
| Depositors： | JJ McCormick |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 2.7660000324249270 Vector: pK (plasmid) Promoters: Promoter lac Construction: pUC19 Marker(s):ampR Construct size (kb): 2.766000032424927 Features: insert detection: lacZ marker(s): ampR promoter: lac replicon: pMB1 |
| Applications： | expression vector vector permitting construction of fusion proteins vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): PvuI--1.8, 1.0; EcoRI--2.8; HindIII--2.8; SspI--2.8; ClaI--2.8. The plasmid contains the following restriction sites (bp from 0): NotI--0; Nsi--75; PvuI--119, 1907; EcoRI--237; HindIII--288; SapI--524; DrdI--749, 2618; BsaI--1607; SspI--2342. Cloning vector with a second multiple cloning site inserted at the KasI site of pUC19 not interrupting lacZ. The order of the major features in this plasmid is: NotI/MCSII/NsiI - 3 lacZ - EcoRI/MCSI/HindIII - 5 lacZ - SapI - pMB1 ori - ampR. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Wang Q, et al. Mammalian expression vectors with modulatable promoters and two multiple cloning sites. Gene 119: 155-161, 1992. PubMed: 1327962 J J McCormick, personal communication |
| Shipped： | freeze-dried |