微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pMLB1034 plasmid in E. coli |
|---|---|
| 商品货号： | TS164048 |
| Designations： | pMLB1034 plasmid in E. coli |
| Depositors： | ML Berman |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.26 Vector: pMLB1034 (plasmid) Promoters: Promoter none Construction: pMC871, pBR322 Marker(s):ampR Construct size (kb): 6.26 Features: insert detection: lacZ marker(s): ampR promoter: none replicon: pMB1 |
| Applications： | promoter-cloning vector vector permitting construction of fusion proteins |
| Comments： | A plasmid vector for screening promoters by determining beta-galactosidase activity (lacZ). Escherichia coli MC1000 = F- araD139 delta(ara, leu)7697 delta(lac)chi74 galU- galK- rpsL. The cloning sites in this vector are 5 to a promoterless beta-galactosidase gene. The insert must provide the promoter, the ribosome binding site, and/or the ATG translation initiation codon for activity to be expressed. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Shultz J, et al. A previously unidentified gene in the spc operon of Escherichia coli K12 specifies a component of the protein export machinery. Cell 31: 227-235, 1982. PubMed: 6297749 Berman ML. Vectors for constructing hybrid genes. BioTechniques 1: 178-183, 1983. |
| Shipped： | freeze-dried |