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| 产品名称： | HFBCC16 |
|---|---|
| 商品货号： | TS164138 |
| Designations： | HFBCC16 |
| GenBank Number： | M78440 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 5.599999904632568 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ, primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: CDC27* Genomic copy number: unique Gene name: cell division cycle 27 (human homolog of S. pombe nuc2+ and A. nidulans bimA) Contains complete coding sequence?: U Chromosome: 17; Localization: 17 q21-q24 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 2.6 Cross references: DNA Seq. Acc.: M78440; EST: EST00588; Primer: M13 Forward Insert lengths(kb): 2.599999904632568 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Gene product: cell division cycle 27 (human homolog of S. pombe nuc2+ and A. nidulans bimA) CDC27* |
| Insert Size (kb)： | 2.600 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.6; PstI--3.0, 1.7, 0.8; HindIII--5.2, 0.3. According to the coding-region prediction program CRM, these sequence ranges (followed by the probability) of EST00588 may contain coding sequence: 101-151 (good). |
| References： | Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749 Tugendreich S, et al. Linking yeast genetics to mammalian genomes: identification and mapping of the human homolog of CDC27 via the expressed sequence tag database. Proc. Natl. Acad. Sci. USA 90: 10031-10035, 1993. PubMed: 8234252 J C Venter, personal communication |