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微信小章| 产品名称： | Herpesvirus saimiri |
|---|---|
| 商品货号： | TS164290 |
| Classification： | Herpesviridae, Rhadinovirus |
| Agent： | Herpesvirus saimiri |
| Strain： | C-488 |
| Common Name： | Herpesvirus saimiri |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | This item contains FBS of Guatemalan origin. Strain is also available as ATCC® VR-1414™, which does not require a USDA permit due to multiple passages of ATCC® VR-1396™ in US origin FBS. Herpesvirus saimiri strain C488 transforms human T lymphocytes to antigen- and mitogen-independent, but IL-2 dependent stable growth. Virus stocks are stable for months at 4C, but titer greatly reduced by freeze-thaw cycles. For methods of propagation see: Fickenscher and Fleckenstein, 1994. in: Methods in Molecular Genetics. (ed) K. W. Adolph, 4: 345-362, 1994. |
| Effect on Host： | CPE, foci of detached cells bordered by rounded enlarged cells |
| Recommended Host： | OMK (ATCC® CRL-1556™)
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| Growth Conditions： | Temperature:xa037°C
Incubation: xa0Incubate infected culture for 10-20 days at 37°C in a humidified 5% CO2xa0atmosphere, until CPE are well advance |
| Name of Depositor： | M Daniel, R Desrosiers, B Biesinger, B Fleckenstein, H Fickenscher |
| Special Collection： | NCRR Contract |
| Source： | Blood lymphocytes of New England Regional Primate Research Ctr. squirrel monkey (Saimiri sciureus) |
| References： | Biesinger B, et al. The divergence between two oncogenic Herpesvirus saimiri strains in a genomic region related to the transforming phenotype. Virology 176: 505-514, 1990. PubMed: 2161148 Desrosiers RC, Falk LA. Herpesvirus saimiri strain variability. J. Virol. 43: 352-356, 1982. PubMed: 6287011 Biesinger B, et al. Stable growth transformation of human T lymphocytes by herpesvirus saimiri. Proc. Natl. Acad. Sci. USA 89: 3116-3119, 1992. PubMed: 1313581 . Methods in Molecular Genetics, Part A. : K.W. Adolph; 1993. |