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| 产品名称： | pHY10 |
|---|---|
| 商品货号： | TS164346 |
| Designations： | pHY10 |
| Species： | Homo sapiens, human |
| Applications： | TaqI and StuI digests seem to reveal polymorphisms but they are too dependent on hybridization conditions for definitive segregation studies. |
| Vector： | Construct size (kb): 9.5600004196167 |
| Insert： | DNA: genomic Insert lengths(kb): 3.563999891281128 Tissue: fibroblast Gene product: DNA Segment, repetitive DYZ1 |
| Insert Size (kb)： | 3.564 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.0, 3.55; BamHI--9.5; HindIII--9.5; AvaI--9.5; PvuII--6.8, 2.7. Contains 64 TaqI sites, 74 HinfI sites, 5 RsaI sites at 507, 822, 2610, 3095, 3176; 2 Sau3AI sites at 575, 2304; 1 HaeIII site at 3526, 1 ClaI site at 1067; and no MspI or SphI sites. Sequence is a tandem repeat of pentanucleotides; 229/713 were TTCCA and 298/713 were single nucleotide substitutions of that: 58-TTCGA, 33-TTCCT, 31-GTCCA, 27-TTCTA, 23-TTCAA, 22-TTGCA, 20-TTTCA. A 3.55 kb band is revealed in EcoRI, HaeIII, and StuI digests. TaqI and StuI digests seem to reveal polymorphisms but they are too dependent on hybridization conditions for definitive segregation studies. |
| References： | Nakahori Y, et al. A human Y-chromosome specific repeated DNA family (DYZ1) consists of a tandem array of pentanucleotides. Nucleic Acids Res. 14: 7569-7580, 1986. PubMed: 3774538 |