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| 产品名称： | Chlamydia trachomatis Serovar Ba |
|---|---|
| 商品货号： | TS164398 |
| Classification： | Chlamydiaceae, Chlamydia |
| Deposited As： | Trachoma serotype ba |
| Agent： | Chlamydia trachomatis Serovar Ba |
| Strain： | Apache-2 |
| Applications： | Sexually Transmitted Disease Research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth.
Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC® CRL-1696™) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus. |
| Effect on Host： | Intracellular inclusion bodies visualized by fluorescent staining |
| Recommended Host： | McCoy cells (ATCC CRL-1696x99) |
| Growth Conditions： | Temperature: 36°C
Recommendations for Infection: For best results, infection should be performed on a 80-100% confluent, 24-48 hour old cellular monolayer.xa0Sonicate seed material for 20 seconds at approximately 240W to disrupt cells.xa0 Prepare dilution of chlamydia in minimum amount of volume (e.g. 1 mL per 25 cm2).xa0 Remove cell growth medium and inoculate with disrupted material. For adsorption, centrifuge at 1500 x g at 25°C for 1 hour.xa0 End adsorption by adding agent growth medium.
Incubation: Incubate infected culture for 72 hours at 36°C in a humidified 5% CO2 atmosphere.
Preservation: Controlled rate freeze. |
| Name of Depositor： | E Jawetz |
| Special Collection： | ATCC |
| Source： | Eye of an Apache Indian child from San Carlos, Arizona |
| References： | Hanna L, et al. Trachoma viruses isolated in United States. I. Growth in embryonated eggs. Proc. Soc. Exp. Biol. Med. 104: 142-145, 1960. PubMed: 14399529 |