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| 产品名称： | SMV-34 |
|---|---|
| 商品货号： | TS164492 |
| Designations： | SMV-34 |
| GenBank Number： | D00717 |
| Species： | Soybean mosaic virus |
| Depositors： | WG Dougherty |
| Vector： | Construct size (kb): 7.699999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 4.5 Gene product: nuclear inclusion proteinase CI |
| Insert Size (kb)： | 4.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.45, 2.15; BamHI--7.6; HindIII--4.55, 2.7, 0.35; EcoRI/HindIII--3.0, 2.25, 1.5, 0.6, 0.35; EcoRI/BamHI--3.2, 2.35, 2.15; PstI/SstI--4.5, 2.8. GenBank accession D00717 corresponds to the 3 end of this insert, which extends approximately from nt 2800 to nt 7300 of the SMV genome. Restriction sites flanking the insert at the 5 end are EcoRI, SacI, and KpnI and at the 3 end are BamHI, XbaI, SalI, PstI, SphI, and HindIII. |
| Classification： | Potyvirus |
| References： | Ghabrial SA, et al. Molecular genetic analyses of the soybean mosaic virus NIa protease. J. Gen. Virol. 71: 1921-1927, 1990. PubMed: 2212987 William G Dougherty, personal communication |