微信小陈
微信小章
产品简介
购买须知
| 产品名称： | E1.4 |
|---|---|
| 商品货号： | TS164566 |
| Designations： | E1.4 |
| Species： | Mus musculus, mouse |
| Depositors： | V Ramamurthy |
| Vector： | Construct size (kb): 4.400000095367432 |
| Insert： | DNA: genomic Insert lengths(kb): 1.399999976158142 Tissue: B-1/200 fibroblast cell line Gene product: adenosine deaminase Ada |
| Insert Size (kb)： | 1.400 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 1.4; HindIII--4.5; BamHI--4.5; PstI--4.5; XbaI--4.5. Contains exon 1 and flanking sequences. Within this clone are sequences which appear to signal termination of transcription, regulating gene expression. The insert contains a SmaI site at nt 79, where nt 1 is the A of the initiation codon. The insert extends from nt -241 to nt +1155. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Ramamurthy V, et al. Sequence requirements for transcriptional arrest in exon 1 of the murine adenosine deaminase gene. Mol. Cell. Biol. 10: 1484-1491, 1990. PubMed: 1690842 Maa MC, et al. Identification of transcription stop sites at the 5 and 3 ends of the murine adenosine deaminase gene. J. Biol. Chem. 265: 12513-12519, 1990. PubMed: 2373702 |