微信小陈
微信小章
产品简介
购买须知
| 产品名称： | Caviid herpesvirus 2 |
|---|---|
| 商品货号： | TS164578 |
| Classification： | Herpesviridae, Unassigned |
| Deposited As： | Deposited as Guinea pig salivary gland virus |
| Agent： | Caviid herpesvirus 2 |
| Strain： | 22122 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Submaxillary glands from a guinea pig |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Effect on Host： | CPE, refractile rounding and sloughing |
| Recommended Host： | Guinea pig lung (ATCC® CCL-158™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 9-21 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% or greater of the monolayer. |
| Name of Depositor： | MJ Collins, Jr., Microbiological Associates, Inc. |
| Source： | Submaxillary glands from a guinea pig |
| References： | Hartley JW, et al. Serial propagation of the guinea pig salivary gland virus in tissue culture. Proc. Soc. Exp. Biol. Med. 96: 281-285, 1957. PubMed: 13485080 |
| Cross References： | Nucleotide (GenBank) : AB592928.1 Caviid herpesvirus 2 DNA, complete genome, strain: 22122 |