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| 产品名称： | M2660 URA3 --> LYS2 converter plasmid in E. coli |
|---|---|
| 商品货号： | TS164620 |
| Designations： | M2660 URA3 --> LYS2 converter plasmid in E. coli |
| Depositors： | DJ Stillman |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 8.308 Vector: M2660 (plasmid) Construction: pUC18 Marker(s):ampR LYS2 Construct size (kb): 8.308 Features: gene disruption cassette: ura3::LYS2 marker(s): ampR replicon: pMB1 restriction site: HindIII |
| Applications： | YI-type (integrating) shuttle vector host modification marker swap vector URA3 --> LYS2 |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglII--4.3, 4.1; HindIII--5.6, 2.8; HpaI--8.5; PvuI--5.1, 2.4, 0.9. To inactivate URA3, transform with HindIII-digested plasmid (5.57 kb) and select for Lys+. A marker swap (URA3->LYS2) vector for altering a Saccharomyces cerevisiae host by a one-step gene disruption. When swapping markers on an episomal plasmid, appropriate phenotype may result from loss of the plasmid unless a second selectable or scorable marker is used to ensure plasmid maintenance. Constructed by replacing an internal EcoRV-PstI fragment of URA3 with a 4.6 kb PvuII-PstI fragment containing LYS2. URA3 and LYS2 are in the same orientation. |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Voth WP, et al. New marker swap plasmids for converting selectable markers on budding yeast gene disruptions and plasmids. Yeast 20: 985-993, 2003. |
| Related Products： | component of:ATCC 87561 |
| Shipped： | frozen |