宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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phpim5R1 [hpim-1, pHPimR1]

货号 TS164675
中文名称 null
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产品名称: phpim5R1 hpim-1, pHPimR1
商品货号: TS164675
Designations: phpim5R1 hpim-1, pHPimR1
Species: Homo sapiens, human
Vector:
Construct size (kb): 6.400000095367432
Insert:
DNA: genomic
Insert lengths(kb): 3.599999904632568
Tissue: 380 cell line
Gene product: proviral integration, MCF PIM
Alleles: A1, A2, B1, B2, C1, A1, A2, B1, B2, C1, C2, C2
Insert Size (kb): 3.600
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 3.05; HindIII--3.6, 2.5, 0.9; PstI--2.45, 1.5, 1.2, 1.1, 0.54; BamHI--5.2, 1.2, 0.48; SalI--7.0.
Insert contains BamHI (2) and HindIII (2) sites.
Includes part of intron 4, exon 5, intron 5, exon 6, and 3 flanking sequences.
Cross-hybridizes to mouse Pim-1 gene under stringent conditions.
Vector PyT is a pBR322 derivative with a deletion between EcoRII sites 1058 and 2500.
Enzyme(s) not detecting polymorphism: EcoRI, HindIII.
References:

Zoghbi HY, et al. Deletion and linkage mapping of eight markers from the proximal short arm of chromosome 6. Genomics 6: 352-357, 1990. PubMed: 1968423

Nagarajan L, et al. Localization of the human pim oncogene (PIM) to a region of chromosome 6 involved in translocations in acute leukemias. Proc. Natl. Acad. Sci. USA 83: 2556-2560, 1986. PubMed: 3458216

Meeker TC, et al. Characterization of the human PIM-1 gene: a putative proto-oncogene coding for a tissue specific member of the protein kinase family. Oncogene Res. 1: 87-101, 1987. PubMed: 3329711