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| 产品名称： | pRML2 |
|---|---|
| 商品货号： | TS164679 |
| Designations： | pRML2 |
| Depositors： | F Spencer |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.0999999046325680
Vector: pRML2 (plasmid)
Promoters: Promoter for in vitro transcription T7
Construction: pBluescript + URA3 + ARSH4
Marker(s): ampR
Features: marker(s): URA3 marker(s): ampR promoter for in vitro transcription: T7 replicon: ARSH4 replicon: ori restriction site: SalI coding sequence: TEL |
| Applications： | cloning vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.1; EcoRI--5.1; NotI--5.1; XbaI--5.1; EcoRI/XbaI--4.5, 0.5. YAC cloning vector. The plasmid was from pBluescript, and includes a URA3 selectable marker, yeast origin of replication ARSH4, a distal polylinker to support plasmid rescue of insert ends, T7 promoter, and a cloning polylinker for library production. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Spencer F, et al. Targeted recombination-based cloning and manipulation of large DNA segments in yeast. Methods 5: 161-175, 1993. Smith DR, et al. Copy number amplification of yeast artifical chromosomes. Methods Enzymol. 216: 603-614, 1992. PubMed: 1336106 Zhong TP, et al. Zebrafish genomic library in yeast artifical chromosomes. Genomics 48: 136-138, 1998. PubMed: AMBIGUOUS 95 Haldi ML, et al. A comprehensive large-insert yeast artificial chromosome library for physical mapping of the mouse genome. Mamm. Genome 7: 767-769, 1996. PubMed: 8854865 |
| Shipped： | freeze-dried |
| Shipping Information： | in E. coli containing the plasmid |