宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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phamSREBP-1

货号 TS164702
中文名称 null
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产品名称: phamSREBP-1
商品货号: TS164702
Designations: phamSREBP-1
GenBank Number:

U09103

Species: Cricetulus griseus, hamster, Chinese
Depositors: JL Goldstein
Vector:

Name of vector: pBluescript KS+
Intact vector size: 2.96
Type of vector: phagemid
Vector end: SalI
Vector end: NotI
Cloning sites: SacII XmaII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI
Construction: pUC19
Host range: Escherichia coli


Features (with orientation and position when available):
enhancer:
none
insert detection: lacZ
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none

Insert:
Genome: hamster, Chinese
Gene symbol: SREBP1*
Gene name: sterol regulatory element binding protein-1
Contains complete coding sequence?: U
Tissue: CHO cell line
Type of DNA: cDNA
Insert end: Modification: SalI linker
Insert end: Modification: NotI adapter
Insert size (kb): 3.83
Cross references: DNA Seq. Acc.: U09103; Nucleotides 1-3816 of the insert correspond to nucleotides 1-3816 of U09103.
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Related Products: Distribution host: Escherichia coli HB101 (ATCC 33694)
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments:
Restriction digests of the clone give the following sizes (kb): SalI/NotI--4.2, 3.0; EcoRI--7.3; HindIII--7.3; PstI--4.4, 1.15, 0.9, 0.4, 0.3; PvuI--5.8, 1.1, 0.4.
References:

Sato R, et al. Assignment of the membrane attachment, DNA binding, and transcriptional activation domains of sterol regulatory binding protein-1 (SREBP-1). J. Biol. Chem. 269: 17267-17273, 1994. PubMed: 8006035