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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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Verticillium dahliae Klebahn

货号 TS164743
中文名称 null
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产品名称: Verticillium dahliae Klebahn
商品货号: TS164743
Deposited As: Verticillium dahliae Klebahn, anamorph
Strain Designations: Brm-1
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format: freeze-dried
Type Strain: no
Preceptrol®: no
Comments:
Melanin biosynthesis
Accumulates (+)-scytalone and flaviolin
Brown microsclerotia
Morphology:

Colonies on potato dextrose agar are grey to olivaceous, powdery or felty. Conidiophores mostly unbranched, with one or few conidial scars, up to 50 µm long, 3-6 µm wide. Conidial chains profusely branched. Conidia obclavate to ellipsoidal, with a short, cylindrical beak, 23-56 x 8-17 µm, medium brown, rugulose with muriform septation, with a single scar at the tip, arising in mostly unbranched chains of ten or more.

Medium: ATCC® Medium 336: Potato dextrose agar (PDA)
Growth Conditions:
Temperature: 24.0°C
Sequenced Data:
xa0xa0xa0xa0 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence.

TATTGATATGCTTAAGTTCAGCGGGTATTCCTACCTGATCCGAGGTCAACCGTTGCCGCACGAGGCGGGCTTGTAGGGGGTTTAGAGGCAAGCGCCTGCGGGACTCCGATGCGAGCTGTAACTACTACGCAAGGAAGGGCCACGCGGGTCCGCCACTGCTTTTAAGGGCCTACAGACGTAGATCCCCAACACCGGGCCACTGGGGCTCGAGGGTTGAAACGACGCTCGGACAGGCATGCCTCCCAGGATACTGGAAGGCGCCATGTGCGTTCAAAGATTCGATGATTCACTGAATTCTGCAATTCACACTACATATCGCGTTTCGCTGCGTTCTTCATCGATGCTAGAGCCAAGAGATCCGTTGTTAAAAGTTTTAATAGTTCGCTAAGAACACTCAGAAGTATCGTTGGTATAAACAGAGAGACTGATGGACCGGCGGGCTCGCAGAACGAGCCGCCGAAGCAACAATATGGTTCACAAAGGGTTATGAGTAGATACTCGGTAATGATCCCTCCGCAG

Name of Depositor: MH Wheeler
Chain of Custody:
ATCC <
Isolation:
UV-induced mutant of ATCC 44574
Cross References:

Nucleotide (GenBank) : JQ070078 ITS including 5.8S rRNA gene

References:

Geis PA, et al. Pentaketide metabolites of melanin synthesis in the dematiaceous fungus Wangiella dermatitidis. Arch. Microbiol. 137: 324-328, 1984. PubMed: 6539583

Wheeler MH, et al. Ultrastructure of melanin formation in Verticillium dahliae with (+)-scytalone as a biosynthetic intermediate. Can. J. Microbiol. 22: 702-711, 1976. PubMed: 945119

Bell AA, et al. Use of mutants to establish (+)-scytalone as an intermediate in melanin biosynthesis by Verticillium dahliae. Can. J. Microbiol. 22: 787-799, 1976. PubMed: 945120

Wheeler MH, et al. Ultrastructural and chemical distinction of melanins formed by Verticillium dahliae from (+)-scytalone, 1,8-dihydroxynaphthalene, catechol, and L-3,4-dihydroxyphenylalanine. Can. J. Microbiol. 24: 289-297, 1978. PubMed: 565670