微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pBH53 |
|---|---|
| 商品货号： | TS164804 |
| Designations： | pBH53 |
| Species： | Homo sapiens, human |
| Depositors： | RL White |
| Applications： | Not recommended for detection of p53 transcripts. The probe was pre-associated with total human DNA. Otherwise, RFLPs were observed under normal hybridization and wash stringencies. |
| Vector： | Construct size (kb): 6.800000190734863 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pUC18 Intact vector size: 2.686 Type of vector: plasmid Vector end: BamHI Vector end: BamHI Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Construction: pUC71K Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ, MCS: HindIII...EcoRI, -> promoter: lac, replicon: pMB1, marker(s): ampR, Cross references: DNA Seq. Acc.: L08752 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: TP53 Genomic copy number: unique Gene name: tumor protein p53 (Li-Fraumeni syndrome) Contains complete coding sequence?: U Chromosome: 17; Localization: 17 p13.1 Type of DNA: genomic Insert end: BamHI Insert end: BamHI Insert size (kb): 4.1 Cross references: Insert lengths(kb): 4.099999904632568 Gene product: tumor protein p53 (Li-Fraumeni syndrome)( tumor protein p53 (Li-Fraumeni syndrome), p53 cellular tumor antigen) TP53 Alleles: D2, C2, C1, A1, A1, B2, B1, D1, A2, A2 |
| Insert Size (kb)： | 4.100 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.1, 2.7; BglI--3.9, 1.1, 0.9, 0.8; EcoRI--6.8; AvaI--3.9, 2.3, 0.5; KpnI--6.8. Not recommended for detection of p53 transcripts. The probe was pre-associated with total human DNA. Otherwise, RFLPs were observed under normal hybridization and wash stringencies. Subcloned from a cosmid isolated by hybridization with a cDNA for p53. Enzymes(s) not detecting polymorphism: BglII, EcoRI, HindIII, MspI, PstI, PvuII, RsaI, TaqI. |
| References： | Hoyheim B, et al. A BamHI-polymorphism is detected by a genomic p53-clone (pBHP53). Nucleic Acids Res. 17: 8898, 1989. PubMed: 2573883 |