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| 产品名称： | pKKM13(-) |
|---|---|
| 商品货号： | TS164805 |
| Designations： | pKKM13(-) |
| Depositors： | R Kreutzer |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.9000000953674320 Vector: pKKM13(-) (phagemid) Promoters: Promoter for expression tac Marker(s):ampR Construct size (kb): 4.900000095367432 Features: marker(s): ampR promoter for expression: tac replicon: M13 replicon: pMB1 MCS: EcoRI...HindIII transcription terminator: T1 transcription terminator: T2 |
| Applications： | expression vector vector permitting production of single-stranded DNA vector useful for site-directed mutagenesis |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.8; BamHI--4.6, 0.2; XbaI--4.8. Expression vector useful for mutagenesis of cloned inserts. Single stranded DNA obtained after superinfection with a suitable M13 helper phage gives the antisense strand with respect to the tac promoter. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Nock S, et al. One-vector system for mutagenesis and over-expression of cloned genes. BioTechniques 18: 608, 1995. PubMed: 7598889 Roland Kreutzer, personal communication |
| Shipped： | freeze-dried |