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| 产品名称： | pNOT19 |
|---|---|
| 商品货号： | TS164916 |
| Designations： | pNOT19 |
| Depositors： | HP Schweizer |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 2.6940000057220460 Vector: pNOT19 (plasmid) Promoters: Promoter lac Construction: pUC19 Marker(s):ampR Construct size (kb): 2.694000005722046 Features: insert detection: lacZ marker(s): ampR promoter: lac replicon: pMB1 |
| Applications： | vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.7; PvuI--1.75, 0.9; HindIII--2.7; NotI--2.7. Available for research purposes only. General purpose cloning vector containing a unique NotI site. Constructed for insertion of mobilization cassettes pMOB1-4 (ATCC 77280-77283) to produce a vector useful for allele replacement in Pseudomonas aeruginosa. Also permits visual detection of recombinants by lacZalpha complementation. The alpha peptide is 107 aa. |
| Media： | ATCC® Medium 1315: LB Medium (ATCC medium 1065) with 100 mg/L ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Schweizer HP. Allelic exchange in Pseudomonas aeruginosa using novel ColE1-type vectors and a family of cassettes containing a portable oriT and the counter-selectable Bacillus subtilis sacB marker. Mol. Microbiol. 6: 1195-1204, 1992. PubMed: 1588818 |
| Shipped： | freeze-dried |