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微信小章| 产品名称： | SNLP 76/7-4 |
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| 商品货号： | TS164933 |
| Organism： | Mus musculus, mouse |
| Tissue： | Embryo |
| Cell Type： | Fibroblast |
| Product Format： | frozen |
| Morphology： | Fibroblast |
| Culture Properties： | Adherent |
| Biosafety Level： | 1 xa0xa0Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: Biosafety in Microbiological and Biomedical Laboratories,xa05th ed. HHS Publication No. (CDC) 93-8395. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.html.
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | embryo |
| Gender： | Male and female mixed |
| Strain： | SIM (Sandos Inbred Mice) |
| Applications： | This cell line can be used as a feeder layer to support the growth of embryonic stem cells PubMed: 17576687; PubMed: 16236760 and induced pluripotent stem (iPS) cells PubMed: 18079707. Inclusion of puromycin in the medium is not necessary for normal cell growth. ATCC tested that this cell line is resistant to:
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| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | SNL76/7, ATCC SCRC-1049™ was clonally-derived from a STO cell line that expresses both G418 resistance and leukaemic inhibitory factor (LIF) at an abundant level PubMed: 2205396.xa0 SNLP 76/7-4, TS164933™ is a puromycin resistant derivative of SNL76/7 PubMed: 17576687. |
| Clinical Data： | Male and female mixed |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.Remove and discard culture medium.xa0
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.xa0 xa0 |
| Cryopreservation： | Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor： | A Bradley |
| Year of Origin： | 1988 |
| References： | McMahon AP, Bradley A. The Wnt-1 (int-1) proto-oncogene is required for development of a large region of the mouse brain. Cell. 62(6): 1073-1085, 1990. PubMed: 2205396 Takahashi K, et al. Induction of pluripotent stem cells from fibroblast cultures. Nat. Protoc. 2(12): 3081-3089, 2007. PubMed: 18079707 Cadiñanos J, Bradley A. Generation of an inducible and optimized piggyBac transposon system. Nucleic Acids Res. 35(12): e87, 2007. PubMed: 17576687 Kist R, et al. Reduction of Pax9 gene dosage in an allelic series of mouse mutants causes hypodontia and oligodontia. Hum. Mol. Genet. 14(23): 3605-3617, 2005. PubMed: 16236760 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo JL. Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988 Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |