微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pIC-ER-F pIC 20H ER 57.4 |
|---|---|
| 商品货号： | TS164980 |
| Designations： | pIC-ER-F pIC 20H ER 57.4 |
| GenBank Number： | M12674 |
| Species： | Homo sapiens, human |
| Depositors： | H Ahrens |
| Applications： | produces protein estrogen receptor |
| Vector： | Construct size (kb): 4.5 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pIC20H Intact vector size: 2.722 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: HindIII SphI PstI SalI XbaI BamHI SmaI KpnI SacI EcoRI ClaI EcoRV BglII XhoI NruI Polylinker sites: HindIII SphI PstI SalI XbaI BamHI SmaI KpnI SacI EcoRI ClaI EcoRV XbaI BglII XhoI SacI NruI HindIII Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ marker(s): ampR replicon: pMB1 Cross references: DNA Seq. Acc.: L08912 |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: ESR Genomic copy number: unique Gene name: estrogen receptor Contains complete coding sequence?: Y Chromosome: 6; Localization: 6 q25.1 Type of DNA: cDNA Insert end: EcoRI Insert end: EcoRI Insert size (kb): 1.8 Cross references: DNA Seq. Acc.: M12674 Insert lengths(kb): 1.799999952316284 Gene product: estrogen receptor ESR Alleles: A1, A2 Target Gene: estrogen receptor |
| Insert Size (kb)： | 1.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 1.8; BglI--2.0, 1.6, 1.15; BglII--4.1, 0.54; HindIII--2.8, 1.0, 0.9; PstI--3.3, 1.0, 0.4, 0.2; NotI--4.6; BamHI--4.5. The insert contains the following restriction sites (approximate kb from the 5 end): BglI--0.47; BglII--1.27; HindIII--1.02; NcoI--1.18; NotI--0.19; PstI--0.36, 1.32, 1.49; PvuII--0.35; SmaI--0.24; StuI--0.62, 0.71, 1.48; XbaI--1.13. Overproduces the fusion protein in E. coli, the majority of which is found in inclusion bodies. It binds estrogens, antiestrogens, and DNA. The recombinant fusion protein contains an additional 23 N-terminal amino acids encoded by the vector polylinker. |
| References： | Ahrens H, et al. Overproduction of full-length and truncated human estrogen receptors in Escherichia coli. Receptor 2: 77-92, 1992. PubMed: 1472947 |