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微信小章| 产品名称： | pE-301 |
|---|---|
| 商品货号： | TS164990 |
| Designations： | pE-301 |
| GenBank Number： | K00396 |
| Species： | Homo sapiens, human |
| Depositors： | JL Breslow |
| Vector： | Construct size (kb): 4.699999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.8999999761581421 Tissue: adult liver Gene product: apolipoprotein E APOE |
| Insert Size (kb)： | 0.900 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--3.8; BamHI, SalI, PvuII or HindIII--4.3. The insert has 5 internal PstI sites. Encodes amino acids 81-299 and contains the 158 bp 3 untranslated region and 44 bp poly(A). The genomic clone lambda APO-E (ATCC 57026, 57027) contains a 14 kb insert extending from 7 kb upstream to 5 kb downstream of the 2 kb EcoRI fragment containing sequences homologous this clone. |
| References： | Breslow JL, et al. Identification and DNA sequence of a human apolipoprotein E cDNA clone. J. Biol. Chem. 257: 14639-14641, 1982. PubMed: 6897404 Breslow JL, et al. Identification and DNA sequence of a human apolipoprotein E cDNA clone. J. Biol. Chem. 258: 14639-14641, 1983. PubMed: 6897404 Zannis VI, et al. Synthesis, intracellular processing, and signal peptide of human apolipoprotein E. J. Biol. Chem. 259: 5495-5499, 1984. PubMed: 6325438 Lusis AJ, et al. Regional mapping of human chromosome 19: organization of genes for plasma lipid transport (APOC1, -C2, and -E and LDLR) and the genes C3, PEPD, and GPI. Proc. Natl. Acad. Sci. USA 83: 3929-3933, 1986. PubMed: 3459164 |