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| 产品名称： | pFH111 |
|---|---|
| 商品货号： | TS165029 |
| Designations： | pFH111 |
| GenBank Number： | XO2761 |
| Species： | Homo sapiens, human |
| Applications： | A 0.5 kb HindIII/BamHI fragment has been used to detect the TaqI polymorphism. |
| Vector： | Construct size (kb): 5.300000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.899999976158142 Tissue: Hs578T cell line Gene product: fibronectin 1 FN1 Alleles: A2, E2, A1, B2, D2, B1, D1, E1 |
| Insert Size (kb)： | 1.900 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): PvuII--4.8, 0.6; HindIII--5.6; BamHI--4.5, 0.7; HindIII/BamHI--3.0, 1.5, 0.7; EcoRI--3.6, 1.5. A 0.5 kb HindIII/BamHI fragment has been used to detect the TaqI polymorphism. The 2.25/2.2 kb TaqI RFLP can be resolved on 1% agarose gels run for 20 cm. Enzyme(s) not detecting polymorphism: HaeIII, HindIII, EcoRI, PstI, BglII. |
| References： | Kornblihtt AR, et al. Primary structure of human fibronectin: differential splicing may generate at least 10 polypeptides from a single gene. EMBO J. 4: 1755-1759, 1985. PubMed: 2992939 Gardella R, et al. A new TaqI RFLP of the human fibronectin (FN1) gene. Nucleic Acids Res. 17: 10520, 1989. PubMed: 2574858 |