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| 产品名称： | pACYC184 |
|---|---|
| 商品货号： | TS165040 |
| Designations： | pACYC184 |
| Depositors： | SN Cohen |
| Biosafety Level： | 1 |
| Host： | Escherichia coli PM191 |
| Vector Information： | Vector size (kb): 4.244 Markers: cmlR, tetR DNA Seq. Acc.: X06403 Type of vector: plasmid Cloning sites: EcoRI HindIII BamHI SalI Construction: p15A, pSC101, Tn9 Host range: Escherichia coli Features): marker(s): cmlR, tetR replicon: p15A Suggested host(s): Escherichia coli chi-1776 (ATCC® 31244™) Escherichia coli chi-1776 (ATCC® 31537™) Escherichia coli chi-1776 (ATCC® 39234™) Escherichia coli chi-1776 (ATCC® 53705™) Escherichia coli C600 (ATCC® 23724™) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; EcoRI/BamHI--2.4, 1.9. Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; EcoRI/BamHI--2.4, 1.9. Low copy number plasmid vector containing the replication system of miniplasmid p15A. |
| Media： | ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol |
| Growth Conditions： | Temperature: 37°C |
| References： | Chang AC, Cohen SN. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J. Bacteriol. 134: 1141-1156, 1978. PubMed: 149110 Rose RE. The nucleotide sequence of pACYC184. Nucleic Acids Res. 16: 355, 1988. PubMed: 3340533 |
| Shipped： | frozen |
| Shipping Information： | Frozen glycerol stock of Escherichia coli containing the plasmid. |