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| 产品名称： | pMF7 BW72 |
|---|---|
| 商品货号： | TS165060 |
| Designations： | pMF7 BW72 |
| Depositors： | M Feiss |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.8000001907348630 Vector: pMF7 (cosmid) Construction: pBR322, lambda Marker(s):ampR Construct size (kb): 6.800000190734863 Features: marker(s): ampR replicon: pMB1 enhancer: none |
| Applications： | vector for constructing genomic libraries |
| Comments： | IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. A general purpose cosmid vector. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Feiss M, et al. Cosmid DNA packaging in vivo. Gene 17: 123-130, 1982. PubMed: 6211394 Feiss M, Widner W. Bacteriophage lambda DNA packaging: scanning for the terminal cohesive end site during packaging. Proc. Natl. Acad. Sci. USA 79: 3498-3502, 1982. PubMed: 6285351 |
| Shipped： | freeze-dried |