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| 产品名称： | pFSEC |
|---|---|
| 商品货号： | TS165094 |
| Designations： | pFSEC |
| Depositors： | EW Miles |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.6799998283386230 Vector: pFSEC (plasmid) Promoters: Promoter Ptrc Construction: pSTR4-2 Marker(s):ampR Construct size (kb): 5.679999828338623 Features: primer site: 5-TTGACAATTAATCATCCGGC-3 promoter: Ptrc terminator: rrnBT1 terminator: rrnBT2 |
| Applications： | cloning vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--uncut; EcoRI--5.6; PstI--5.6; EcoRI/PstI--4.4, 1.3. Overproduces yeast cystathionine beta-synthase (full length). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Jhee KH, et al. Domain architecture of the heme-independent yeast cystathionine beta-synthase provides insights into mechanisms of catalysis and regulation. Biochemistry 39: 10548-10556, 2000. PubMed: 10956046 Jhee KH, et al. Yeast cystathionine beta-synthase is a pyridoxal phosphate enzyme but, unlike the human enzyme, is not a heme protein. J. Biol. Chem. 275: 11541-11544, 2000. PubMed: 10766767 |
| Related Products： | component of:ATCC 87809 |
| Shipped： | freeze-dried |
| Shipping Information： | xa0Freeze dried E. coli containing the plasmid. |