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| 产品名称： | pbetaAct-CAT 9 |
|---|---|
| 商品货号： | TS165100 |
| Designations： | pbetaAct-CAT 9 |
| Species： | Gallus gallus, chicken |
| Depositors： | N Fregien |
| Vector： | Construct size (kb): 7.0 |
| Insert： | DNA: genomic Insert lengths(kb): 4.300000190734863 Gene product: actin, beta ACTB |
| Insert Size (kb)： | 4.300 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--7.0; HindIII--4.3, 2.7; XhoI--7.0. The 2.7 kb HindIII fragment contains the actin promoter and the partial actin genomic DNA. The 4.3 kb HindIII fragment contains the CAT gene, the SV40 splicing and polyadenylation sequences and the pUC18 vector. Contains the beta-actin promoter with its CAAT and TATA sequences, 1.5 kb of upstream flanking sequence, the transcription initiation signal, exon 1 (untranslated), intron 1, and a small segment of exon 2. SV40 promoter sequence deleted. |
| References： | Fregien N, Davidson N. Activating elements in the promoter region of the chicken beta-actin gene. Gene 48: 1-11, 1986. PubMed: 3470237 |