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| 产品名称： | pAcMP3/PKCeta |
|---|---|
| 商品货号： | TS165126 |
| Designations： | pAcMP3/PKCeta |
| Species： | Homo sapiens, human |
| Depositors： | DJ Burns |
| Applications： | in another host, produces protein protein kinase C, eta |
| Vector： | Construct size (kb): 12.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pAcMP3 Intact vector size: Type of vector: plasmid Vector end: XbaI Vector end: XbaI Cloning sites: Polylinker sites: BamHI XbaI EcoRI NotI EagI PstI BglII Host range: insect cells; Escherichia coli Features (with orientation and position when available): promoter: basic protein, -> marker(s): ampR replicon: pMB1 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: PRKCH Genomic copy number: unique Gene name: protein kinase C, eta Contains complete coding sequence?: Y Tissue: keratinocytes Type of DNA: cDNA Insert end: NheI Insert end: NheI Insert size (kb): 2.0 Cross references: Insert lengths(kb): 2.0 Tissue: keratinocytes Gene product: protein kinase C, eta PRKCH Target Gene: protein kinase C, eta |
| Insert Size (kb)： | 2.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--10.0, 1.3, 0.55; BglII--11.0, 0.9; PvuI--6.0, 4.0, 1.5, 0.95; PvuII--3.2, 3.0, 2.4, 1.8, 1.4, 0.45; SacI--12.0. The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--1.21, 1.75; BglII--1.43; PvuI--1.28; PvuII--0.12, 1.86; SacI--1.37. Constructed using NheI adaptors. Most of the insert (all but approximately 250 bp) can be excised using BamHI, but the insert will be in two fragments- 1.2 and 0.55 kb. Contains the complete coding sequence only. |
| References： | Rice JW, et al. A comparison of large-scale Sf9 insect cell growth and protein production: stirred vessel vs. airlift. BioTechniques 15: 1052-1059, 1993. PubMed: 8292338 David J Burns, personal communication |