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| 产品名称： | pBG1 |
|---|---|
| 商品货号： | TS165174 |
| Designations： | pBG1 |
| Depositors： | KL Gould |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 5.6999998092651370 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pUC18 Intact vector size: 2.686 Type of vector: cosmid Vector end: NdeI Vector end: NdeI Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Construction: pUC71K Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ, MCS: HindIII...EcoRI, -> promoter: lac, replicon: pMB1, marker(s): ampR, Cross references: DNA Seq. Acc.: L08752 Vector: pBG1 (plasmid) Promoters: Promoter lac Construction: pUC18, ars1, his3 Marker(s):ampR,his3 Construct size (kb): 5.699999809265137 Features: insert detection: lacZ marker(s): ampR marker(s): his3+ promoter: lac replicon: ars1 replicon: pMB1 MCS: SacI...SphI |
| Applications： | contains sequence autonomous replication sequence contains sequence imidazole acetol phosphate transaminase shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.2, 0.55; DraI/EcoRI--2.5, 2.4, 0.7; HindIII--3.5, 2.2; PstI--5.7. The his3 insert contains the following restriction sites (approximate kb from the 5 end): BamHI--0.49; ClaI--0.04; EcoRI--0.47, 1.01; HindIII--1.03; PvuI--0.04; XbaI--0.27; XhoI--0.84. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Burke JD, Gould KL. Molecular cloning and characterization of the Schizosaccharomyces pombe his3 gene for use as a selectable marker. Mol. Gen. Genet. 242: 169-176, 1994. PubMed: 8159167 |
| Related Products： | component of:ATCC 87686 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |