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| 产品名称： | pSE1112 |
|---|---|
| 商品货号： | TS165237 |
| Designations： | pSE1112 |
| Species： | Homo sapiens, human |
| Depositors： | SJ Elledge |
| Applications： | in another host, produces protein SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase |
| Vector： | DESCRIPTION OF VECTOR COMPONENT: Name of vector: pAS1 Intact vector size: 7.100 Type of vector: phagemid Vector end: BamHI Vector end: BamHI Cloning sites: NdeI NcoI SfiI SmaI BamHI SalI Polylinker sites: NdeI NcoI SfiI SmaI BamHI SalI Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli Features (with orientation and position when available): promoter: ADC1 (ADH), -> other: GAL4 DNA-binding domain, -> epitope tag: hemagglutinin (HA), -> restriction site: EcoRI, ->, 4315 MCS: NdeI...SalI, ->, 4395-4422 terminator: ADC1 replicon: pMB1 marker(s): ampR replicon: 2 micron marker(s): TRP1, -> replicon: f1 Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: human Gene symbol: SNF1 Gene name: SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase Contains complete coding sequence?: U Type of DNA: genomic Insert end: BamHI Insert end: BamHI Insert size (kb): 2.3 Cross references: Insert lengths(kb): 2.299999952316284 Gene product: SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase SNF1 Target Gene: SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase |
| Insert Size (kb)： | 2.300 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--9.4; HindIII--5.8, 3.4; BamHI--7.0, 2.3. pSE1111 (ATCC 87010) and pSE1112 (TS165237) can be used together as a positive control in 2-hybrid cloning systems. Expresses SNF1 fused to the GAL4 DNA-binding domain. |
| References： | Durfee T, et al. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev. 7: 555-569, 1993. PubMed: 8384581 Stephen J Elledge, personal communication |