| 产品名称: | pDP1284 |
|---|---|
| 商品货号: | TS165256 |
| Designations: | pDP1284 |
| GenBank Number: | M58458 |
| Species: | Homo sapiens, human |
| Applications: | Sequence analysis and mapping data suggest that RPS4X is the same as the previously identified SCAR. RPS4X maps distal to the breakpoint in A68-2A and proximal to the breakpoint in W4-1A. |
| Vector: | Construct size (kb): 3.799999952316284 |
| Insert: | DNA: cDNA Insert lengths(kb): 0.8999999761581421 Tissue: lymphoblastoid OXEN cell line (49, XYYYY) Gene product: ribosomal protein S4, X-linked RPS4X |
| Insert Size (kb): | 0.900 |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments: | Restriction digests of the clone give the following sizes (kb): NotI--2.9, 0.9; PvuI--2.85, 1.1; PvuII--2.6, 1.3; KpnI--3.8; SacI--3.8. Expression of RPS4X is not dosage-compensated. Sequence analysis and mapping data suggest that RPS4X is the same as the previously identified SCAR. RPS4X maps distal to the breakpoint in A68-2A and proximal to the breakpoint in W4-1A. The insert contains the complete coding sequence for a polypeptide of 263 amino acids, 35 nucleotides 5 untranslated and 61 nucleotides 3 untranslated followed by 10 adenosines. pDP1284 detects an abundant 1.0 kb transcript in male and female cells. pDP1278 (ATCC 65108, 65109) and pDP1284 (ATCC 65110, 65111) each detect a larger number of restriction fragments in Southern blots than expected from either locus alone. There are probably pseudogenes. |
| References: | Fisher EM, et al. Homologous ribosomal protein genes on the human X and Y chromosomes: escape from X inactivation and possible implications for Turner syndrome. Cell 63: 1205-1218, 1990. PubMed: 2124517 |